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Development of an in planta system to monitor phosphorus status by agroinfiltration and agroinjection 2019/05/28

Insufficient supply of phosphate (Pi) fertilizer will limit crop growth and reduce yield. On the other hand, apply excessive amounts of Pi fertilizer will increase cost, accumulation of salt in the soil, and cause the eutrophication of lakes and streams. In order to maintain crop yield and agricultural sustainability, it is necessary to apply Pi fertilizer properly. Therefore, an accurate monitoring system is needed to evaluate the demand for Pi fertilizer in crops. We used agroinfiltration and agroinjection techniques to transiently expressed the GUS reporter gene driven by the TPSI1 promoter (a phosphate starvation-induced gene) in tobacco leaves and tomato fruits. It can be observed that the GUS staining results truly reflect the Pi content in the tobacco leaves (Fig. 1A) and tomato fruits (Fig. 1B). Moreover, the agroinfiltration/agroinjection-based diagnostic systems are rapid, specific, simple, convenient, and do not require expensive equipment.

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(B)


 

Fig. 1. Determination of phosphate content in tobacco leaves (A) and tomato fruits (B) by agroinfiltration and agroinjection. Tobacco and tomato plants were grown under Pi-sufficient (+Pi) conditions or subjected to Pi-deficient (−Pi) and Pi-resupply (Re) treatments for different days (D).